Reprospher 100 4-EP

4-Ethylpyridine-Modified HPLC Column for Enhanced Retention of Basic and Polar Compounds

Reprospher 100 4-EP is a reversed-phase HPLC column from Dr. Maisch HPLC GmbH, packed with ultra-pure, fully porous spherical silica (100 Å pore size) bonded with 4-ethylpyridine (4-EP) ligands. The phase combines hydrophobic interactions with a basic surface character, offering enhanced peak shape and retention for basic, polar, and aromatic compounds, particularly those prone to tailing on conventional C18 columns. The 4-ethylpyridine functionality provides a more accessible basic surface compared to alkyl phases, improving analyte interaction control and increasing selectivity for method development.

Reprospher 100 4-EP from Dr. Maisch HPLC GmbH offers distinctive reversed-phase performance with a basic character – ideal for laboratories that require improved peak symmetry and alternative selectivity for polar and basic compounds.

Key Features:

  • 4-Ethylpyridine bonded phase on 100 Å spherical silica

  • Improved peak shape for basic and ionizable analytes

  • Enhanced selectivity for polar and aromatic compounds

  • High-purity, fully porous silica for robust and reproducible performance

  • Manufactured and quality-controlled by Dr. Maisch HPLC GmbH

Typical Applications:

  • Separation of basic pharmaceuticals, amines, and polar drugs

  • Method development for structurally similar polar analytes

  • LC-MS-compatible reversed-phase workflows

  • Selectivity enhancement compared to traditional C18 columns

Specifications

Request Product
Particle Sizes
  • 5 µm
  • 10 µm
Pore Size 100 Å
Surface Area 350 m²/g
Phase/Modification 4-Ethylpyridin
Particle Type
Separation Modes
Endcapping No
Carbonload 8 %
pH Range 2 to 8

Material Numbers

Phase Material Number
Reprospher 100 4-EP, 5 µm rs15.4ep.
Reprospher 100 4-EP, 10 µm rs10.4ep.
Reprospher 100 2-EP Reprospher 100 Biphenyl

Any questions?
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info@dr-maisch.com +49 7073 5035 7

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