Reprospher 100 C18/WCX is a mixed-mode HPLC column from Dr. Maisch HPLC GmbH, based on high-purity, fully porous silica with a 100 Å pore size. This stationary phase features a dual functionality: a classical C18 ligand for hydrophobic reversed-phase interaction and a weak cation-exchange (WCX) group for electrostatic retention of basic analytes. The combination of both mechanisms provides enhanced selectivity, improved resolution of structurally similar compounds, and excellent peak shape for ionizable substances. This column is particularly well suited for the separation of basic drug substances, polar analytes, and metabolites — especially in LC-MS compatible workflows.
Reprospher 100 C18/WCX from Dr. Maisch HPLC GmbH offers powerful mixed-mode selectivity – ideal for resolving basic and polar analytes in complex reversed-phase HPLC separations.
C18 bonded phase with integrated weak cation-exchange functionality
Fully porous silica with 100 Å pore size
Dual retention mechanisms: hydrophobic and electrostatic
Excellent peak shape and selectivity for basic compounds
Available in 3 µm, 5 µm, and 10 µm particle sizes
Basic pharmaceutical substances and their metabolites
LC-MS compatible reversed-phase separations with improved control
Method development requiring retention of ionizable compounds
Analysis of structurally related bases or polar drugs
| Particle Sizes |
|
|---|---|
| Pore Size | 100 Å |
| Surface Area | 350 m²/g |
| Phase/Modification | C18-COOH |
| Particle Type | |
| Separation Modes | |
| USP Code | |
| Endcapping | No |
| Carbonload | 17 % |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| Reprospher 100 C18-WCX, 3 µm | rs13.9ac. |
| Reprospher 100 C18-WCX, 5 µm | rs15.9ac. |
| Reprospher 100 C18-WCX, 10 µm | rs10.9ac. |