Reprospher 100 C4 is a reversed-phase HPLC column from Dr. Maisch HPLC GmbH, based on high-purity, fully porous silica with a 100 Å pore size and bonded with butyl ligands. The shorter alkyl chain provides reduced hydrophobicity compared to C18 or C8 phases, making this column ideal for the analysis of large, moderately hydrophobic compounds such as peptides, small proteins, and lipophilic pharmaceuticals. Its lower retention strength facilitates faster elution of strongly retained compounds, supports gradient optimization, and offers complementary selectivity in reversed-phase method development.
Reprospher 100 C4 from Dr. Maisch HPLC GmbH is a robust reversed-phase column that offers optimized selectivity for moderately hydrophobic and large analytes – ideal for gradient separations and peptide profiling in pharmaceutical and biochemical analysis.
Butyl-bonded C4 phase – USP L26 classification
Fully porous, high-purity silica with 100 Å pore size
Lower hydrophobicity for shorter retention times
Excellent peak shape for peptides, small proteins, and lipophilic molecules
High batch-to-batch reproducibility
Available in 1.8 µm, 3 µm, and 5 µm particle sizes
Peptides, peptide fragments, and small proteins
Lipophilic drug substances and metabolites
Fast reversed-phase separations of highly retained compounds
Orthogonal selectivity in method scouting
| Particle Sizes |
|
|---|---|
| Pore Size | 100 Å |
| Surface Area | 350 m²/g |
| Phase/Modification | C4 |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | Yes |
| Carbonload | 6 % |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| Reprospher 100 C4, 1.8 µm | rs118.4e. |
| Reprospher 100 C4, 3 µm | rs13.4e. |
| Reprospher 100 C4, 5 µm | rs15.4e. |