Reprospher 100 C4-Aqua

Aqueous-Compatible C4 Column for Polar and Hydrophobic Analytes

Reprospher 100 C4-Aqua is a specially modified reversed-phase HPLC column from Dr. Maisch HPLC GmbH, designed for robust operation under 100% aqueous conditions. Based on high-purity, fully porous silica with a 100 Å pore size, this column is bonded with short-chain butyl ligands and an embedded polar functionality that enhances wetting and retention of polar compounds. Thanks to its unique Aqua-compatible surface chemistry, the column maintains stable retention and excellent peak shape for polar and moderately hydrophobic analytes, even in highly aqueous mobile phases — ideal for LC-MS, bioanalytical, and pharmaceutical applications.

Reprospher 100 C4-Aqua from Dr. Maisch HPLC GmbH combines aqueous compatibility with short-chain reversed-phase selectivity – a smart solution for polar analytes and water-rich HPLC separations.

Key Features:

  • Butyl C4 phase with embedded polar functionality – suitable for 100% aqueous eluents

  • Fully porous silica with 100 Å pore size

  • Enhanced retention of polar analytes in water-rich mobile phases

  • Suppressed silanol interactions and excellent peak symmetry

  • Available in 5 µm, 10 µm and 30 µm particle sizes

Typical Applications:

  • Reversed-phase separations under highly aqueous conditions

  • Polar drugs, peptides, and hydrophilic metabolites

  • LC-MS compatible methods without ion-pairing reagents

  • Bioanalytical and pharmaceutical method development

Specifications

Request Product
Particle Sizes
  • 5 µm
  • 10 µm
  • 30 µm
Pore Size 100 Å
Surface Area 350 m²/g
Phase/Modification C4
Particle Type
Separation Modes
USP Codes
Endcapping Yes
Carbonload 6 %
pH Range 2 to 8

Material Numbers

Phase Material Number
Reprospher 100 C4-Aqua, 5 µm rs15.4aq.
Reprospher 100 C4-Aqua, 10 µm rs10.4aq.
Reprospher 100 C4-Aqua, 30 µm rs130.4aq.
Reprospher 100 C4 Reprospher 100 C4-DE

Any questions?
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info@dr-maisch.com +49 7073 5035 7

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