Reprospher 200 C18-Aqua is a reversed-phase HPLC column from Dr. Maisch HPLC GmbH, based on high-purity, fully porous silica with a 200 Å pore size and bonded with octadecyl ligands featuring a polar surface modification. This design prevents phase collapse under highly aqueous conditions and enhances retention of polar or hydrophilic biomolecules such as peptides and small proteins. With strong hydrophobic retention and excellent peak symmetry, this column is ideal for LC-MS-compatible methods requiring 100% water or high water content in the mobile phase.
This wide-pore Aqua-compatible phase from Dr. Maisch HPLC GmbH ensures robust, reproducible separations of polar biomolecules under aqueous conditions – ideal for challenging reversed-phase applications in life sciences and bioanalysis.
C18 bonded phase with Aqua-modified surface – USP L1 classification
Wide-pore (200 Å) silica for peptides, proteins, and hydrophilic macromolecules
Stable performance in 100% aqueous eluents
Enhanced retention of polar analytes and minimal peak tailing
Available in 3 µm and 5 µm particle sizes
Peptides, polar proteins, and small hydrophilic biomolecules
Bioanalytical workflows with high water content
LC-MS methods for water-soluble pharmaceutical substances
Method development for early-eluting compounds in reversed-phase HPLC
| Particle Sizes |
|
|---|---|
| Pore Size | 200 Å |
| Surface Area | 160 m²/g |
| Phase/Modification | C18 |
| Particle Type | |
| Separation Mode | |
| USP Codes | |
| Endcapping | Yes |
| Carbonload | 5 % |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| Reprospher 200 C18-Aqua, 3 µm | rs23.9aq. |
| Reprospher 200 C18-Aqua, 5 µm | rs25.9aq. |