Reprospher 300 C18 is a reversed-phase HPLC column from Dr. Maisch HPLC GmbH, based on fully porous, high-purity silica with a 300 Å pore size and bonded with octadecyl (C18) ligands. The extra-wide pores allow efficient mass transfer and analyte accessibility, making this phase ideal for the separation of intact proteins, monoclonal antibodies, peptides, and other high-molecular-weight biomolecules. With strong hydrophobic retention, high mechanical stability, and excellent peak symmetry, this column supports robust bioanalytical and pharmaceutical workflows.
This 300 Å wide-pore phase from Dr. Maisch HPLC GmbH is optimized for high-performance separations of large biomolecules — ideal for modern bioanalysis and therapeutic protein characterization.
Octadecylsilane (C18) bonded phase – USP L1 classification
Fully porous silica with 300 Å pore size for large biomolecules
Strong hydrophobic interaction and high column efficiency
Excellent resolution of proteins, peptides, and aggregates
Available from 1.8 µm to 10 µm particle sizes
Intact proteins, monoclonal antibodies, and enzyme fragments
Large peptides, glycoproteins, and aggregates
Reversed-phase LC-MS and bioanalytical separations
Method development for large-molecule therapeutics
| Particle Sizes |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | C18 |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | Yes |
| Carbonload | 7 % |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| Reprospher 300 C18, 1.8 µm | rs318.9e. |
| Reprospher 300 C18, 2 µm | rs32.9e. |
| Reprospher 300 C18, 3 µm | rs33.9e. |
| Reprospher 300 C18, 5 µm | rs35.9e. |
| Reprospher 300 C18, 10 µm | rs30.9e. |