Reprospher 300 C8-TN is a reversed-phase HPLC column from Dr. Maisch HPLC GmbH, packed with ultra-pure, fully porous spherical silica (300 Å pore size) functionalized with trifunctionally bonded octyl ligands and deliberately not endcapped. This design provides moderate hydrophobic retention from the C8 chains, while the absence of endcapping leaves residual silanol groups active, enabling additional polar and ionic interactions. With its wide 300 Å pore size, this stationary phase is particularly well suited for large biomolecules such as peptides, proteins, and oligonucleotides. The mixed-mode retention behavior makes Reprospher 300 C8-TN highly valuable for method development and for analytes that require enhanced interaction beyond hydrophobic retention alone.
Reprospher 300 C8-TN from Dr. Maisch HPLC GmbH delivers robust and versatile performance – the ideal column for laboratories seeking mixed-mode selectivity and reliable separation of large biomolecules.
Trifunctional C8 bonding without endcapping – USP L7
300 Å pore size optimized for peptides, proteins, and biomolecules
Moderate hydrophobic retention with silanol-driven polar selectivity
Mixed-mode retention for challenging analytes
Manufactured and QC-tested by Dr. Maisch HPLC GmbH
Available in 5 µm
Separation of peptides, proteins, and oligonucleotides
Mixed-mode HPLC of polar and basic biomolecules
Method development requiring controlled silanol activity
Gradient and isocratic separations in bioanalytical workflows
| Particle Size |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | C8 |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | No |
| Carbonload | 5 % |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| Reprospher 300 C8-TN, 5 µm | rs35.8tn. |