Reprospher 300 C18-Aqua is a reversed-phase HPLC column from Dr. Maisch HPLC GmbH, packed with fully porous, high-purity silica featuring a 300 Å pore size and octadecyl (C18) bonding with Aqua-compatible surface modification. This phase is specially designed for use with 100% aqueous mobile phases, preventing phase collapse and ensuring stable retention of large, hydrophilic, or polar biomolecules such as peptides, glycoproteins, and biologics. Its combination of wide pore size and polar-modified surface offers excellent mass transfer, reproducible peak shapes, and reliable performance under water-rich conditions.
This Aqua-compatible wide-pore phase from Dr. Maisch HPLC GmbH delivers dependable retention and resolution for large, polar biomolecules – ideal for reversed-phase HPLC under highly aqueous conditions.
C18 bonded phase with Aqua modification for 100% aqueous compatibility
Fully porous silica with 300 Å pore size – ideal for large biomolecules
Excellent retention and peak shape for hydrophilic peptides and proteins
Stable reversed-phase performance without phase collapse
Available in 3 µm and 5 µm particle sizes
Polar peptides, glycopeptides, and hydrophilic proteins
LC-MS workflows with high water content in mobile phases
Bioanalytical and pharmaceutical method development
Reversed-phase separations requiring large-pore, aqueous-stable performance
| Particle Sizes |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | C18 |
| Particle Type | |
| Separation Modes | |
| USP Codes | |
| Endcapping | Yes |
| Carbonload | 4 % |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| Reprospher 300 C18-Aqua, 3 µm | rs33.9aq. |
| Reprospher 300 C18-Aqua, 5 µm | rs35.9aq. |