Reprospher 300 C18-DE

Double Endcapped C18 Reversed-Phase Column for Large Biomolecules

Reprospher 300 C18-DE is a high-performance reversed-phase HPLC column from Dr. Maisch HPLC GmbH, packed with ultra-pure, fully porous spherical silica (300 Å pore size) bonded with octadecyl ligands and double endcapped for maximum deactivation of residual silanol groups. This advanced surface treatment ensures excellent peak symmetry and reproducibility, particularly for basic and polar analytes, while maintaining strong hydrophobic retention. The wide 300 Å pore structure is optimized for the analysis of peptides, proteins, and other large biomolecules, allowing efficient mass transfer and reliable recovery of high-molecular-weight compounds. The double endcapping provides superior stability and column lifetime in demanding reversed-phase applications.

Reprospher 300 C18-DE from Dr. Maisch HPLC GmbH delivers robust and reliable performance – the ideal choice for laboratories analyzing large biomolecules and complex pharmaceutical samples where peak symmetry and stability are essential.

Key Features:

  • C18 reversed-phase silica with double endcapping – USP L1

  • 300 Å pore size ideal for peptides, proteins, and biomolecules

  • Excellent peak shape for basic and polar analytes

  • Strong hydrophobic retention with high reproducibility

  • Manufactured and QC-tested by Dr. Maisch HPLC GmbH

  • Available in 2.5 µm, 3 µm and 5 µm particle sizes

Typical Applications:

  • Reversed-phase HPLC of peptides, proteins, and oligonucleotides

  • Bioanalytical workflows for therapeutic biomolecules

  • Gradient separations with reproducible retention of large analytes

  • Pharmaceutical QC and stability-indicating methods

Specifications

Request Product
Particle Sizes
  • 2.5 µm
  • 3 µm
  • 5 µm
Pore Size 300 Å
Surface Area 100 m²/g
Phase/Modification C18
Particle Type
Separation Mode
USP Code
Endcapping Yes
Carbonload 7 %
pH Range 2 to 8

Material Numbers

Phase Material Number
Reprospher 300 C18-DE, 2.5 µm rs325.9de.
Reprospher 300 C18-DE, 3 µm rs33.9de.
Reprospher 300 C18-DE, 5 µm rs35.9de.
Reprospher 300 C18-Aqua Reprospher 300 C18-T

Any questions?
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info@dr-maisch.com +49 7073 5035 7

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