Reprospher 300 C18-TN is a reversed-phase HPLC column from Dr. Maisch HPLC GmbH, packed with ultra-pure, fully porous spherical silica (300 Å pore size) functionalized with trifunctionally bonded octadecyl ligands and deliberately not endcapped. This design provides strong hydrophobic retention via the C18 chains, while the absence of endcapping maintains active silanol groups, enabling polar and ionic interactions in addition to standard reversed-phase retention. The wide 300 Å pore size is optimized for large biomolecules such as peptides, proteins, and oligonucleotides, ensuring efficient mass transfer and recovery. The combination of hydrophobic and silanol-driven interactions makes Reprospher 300 C18-TN especially valuable for method development where alternative selectivity is required.
Reprospher 300 C18-TN from Dr. Maisch HPLC GmbH delivers robust reversed-phase performance with enhanced selectivity options – the ideal column for laboratories requiring mixed-mode retention and reliable separation of biomolecules.
Trifunctional C18 bonding without endcapping – USP L1
300 Å pore size for proteins, peptides, and oligonucleotides
Mixed-mode retention: hydrophobic + polar/ionic interactions
Robust bonding chemistry ensures stability and reproducibility
Manufactured and QC-tested by Dr. Maisch HPLC GmbH
Available in 5 µm and 30 µm particle sizes
Separation of peptides, proteins, and large biomolecules
Mixed-mode chromatography of polar and basic compounds
Method development requiring controlled silanol activity
Gradient and isocratic separations of complex samples
| Particle Sizes |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | C18 |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | No |
| Carbonload | 10 % |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| Reprospher 300 C18-TN, 5 µm | rs35.9tn. |
| Reprospher 300 C18-TN, 30 µm | rs330.9tn. |