Reprospher 300 PEI is a strong anion exchange (SAX) column from Dr. Maisch HPLC GmbH, based on fully porous, high-purity silica with a 300 Å pore size and coated with polyethyleneimine. This polymeric modification introduces multiple positively charged sites, providing high ion-exchange capacity and excellent selectivity for negatively charged analytes such as nucleotides, oligonucleotides, phosphorylated peptides, and acidic proteins. The wide-pore structure ensures efficient mass transfer and retention of high-molecular-weight analytes under various ionic-strength conditions.
This wide-pore SAX phase from Dr. Maisch HPLC GmbH combines high loading capacity with robust selectivity — ideal for separating large, negatively charged biomolecules in complex matrices.
Polyethyleneimine (PEI) coating for strong anion-exchange retention – comparable to USP L14
Fully porous silica with 300 Å pore size for large, acidic biomolecules
High capacity and stable performance across a wide pH and salt range
Suitable for LC-MS and UV detection of anionic species
Available in 3 µm, 5 µm and 10 µm particle sizes
Oligonucleotides, phosphorylated peptides, and acidic proteins
Ion-exchange chromatography of bioactive compounds
Method development for strong anion exchangers in biopharmaceutical workflows
Preparative and analytical SAX separations in life sciences
| Particle Sizes |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | PEI |
| Particle Type | |
| Separation Modes | |
| Endcapping | No |
| Carbonload | 4 % |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| Reprospher 300 PEI, 3 µm | rs33.pei. |
| Reprospher 300 PEI, 5 µm | rs35.pei. |
| Reprospher 300 PEI, 10 µm | rs30.pei. |