Reprospher 300 C4-DE is a high-performance reversed-phase HPLC column from Dr. Maisch HPLC GmbH, packed with ultra-pure, fully porous spherical silica (300 Å pore size) bonded with butyl chains and double endcapped to minimize residual silanol activity. The short-chain C4 bonding provides mild hydrophobic retention, making this phase particularly suitable for the separation of large biomolecules such as proteins, glycoproteins, and peptides, which require lower hydrophobic interaction for optimal recovery. The wide 300 Å pore size ensures efficient mass transfer for high-molecular-weight analytes, while the double endcapping guarantees excellent peak symmetry, reproducibility, and stability in gradient and isocratic modes.
Reprospher 300 C4-DE from Dr. Maisch HPLC GmbH delivers reliable performance and high recovery – the ideal column for laboratories working with proteins and large biomolecules in advanced reversed-phase HPLC.
C4 short-chain reversed-phase silica with double endcapping – USP L26
300 Å pore size optimized for proteins and large biomolecules
Mild hydrophobic retention for better recovery of sensitive analytes
Excellent peak symmetry for peptides and intact proteins
Manufactured and QC-tested by Dr. Maisch HPLC GmbH
Available in 2.5 µm, 3 µm, and 5 µm particle sizes
Reversed-phase HPLC of proteins, glycoproteins, and peptides
Bioanalytical separations of therapeutic biomolecules
Gradient methods for large hydrophobic compounds
Stability-indicating assays in biopharmaceutical analysis
| Particle Sizes |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | C4 |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | Yes |
| Carbonload | 3 % |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| Reprospher 300 C4-DE, 2.5 µm | rs325.4de. |
| Reprospher 300 C4-DE, 3 µm | rs33.4de. |
| Reprospher 300 C4-DE, 5 µm | rs35.4de. |