Reprospher 300 C4-T

Trifunctional C4 Reversed-Phase Column for Robust Protein and Biomolecule Separations

Reprospher 300 C4-T is a high-performance reversed-phase HPLC column from Dr. Maisch HPLC GmbH, packed with ultra-pure, fully porous spherical silica (300 Å pore size) functionalized with trifunctionally bonded butyl ligands. The short-chain C4 bonding provides mild hydrophobic retention, making it particularly suitable for large biomolecules such as proteins, glycoproteins, and peptides, while the trifunctional bonding chemistry ensures enhanced phase stability and long column lifetime. The wide pore size of 300 Å allows efficient mass transfer and accessibility for high-molecular-weight analytes, ensuring high recovery and reproducible peak shapes even in complex bioanalytical workflows.

Reprospher 300 C4-T from Dr. Maisch HPLC GmbH provides reliable performance, excellent recovery, and robust phase stability – the ideal column for laboratories focused on proteins and large biomolecules in advanced reversed-phase HPLC.

Key Features:

  • Trifunctional C4 reversed-phase bonding – USP L26

  • 300 Å pore size optimized for proteins and large biomolecules

  • Mild hydrophobic retention for sensitive biomolecule analysis

  • High chemical and mechanical stability for robust performance

  • Manufactured and QC-tested by Dr. Maisch HPLC GmbH

  • Available in 5 µm

Typical Applications:

  • Reversed-phase HPLC of proteins, glycoproteins, and peptides

  • Bioanalytical separations of therapeutic biomolecules

  • Gradient separations of high-molecular-weight analytes

  • Stability-indicating assays in pharmaceutical and biopharmaceutical analysis

Specifications

Request Product
Particle Size
  • 5 µm
Pore Size 300 Å
Surface Area 100 m²/g
Phase/Modification C4
Particle Type
Separation Mode
USP Code
Endcapping Yes
Carbonload 4 %
pH Range 2 to 8

Material Number

Phase Material Number
Reprospher 300 C4-T, 5 µm rs35.4t.
Reprospher 300 C4-DE Reprospher 300 C4-TN

Any questions?
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info@dr-maisch.com +49 7073 5035 7

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