Reprospher 300 C4-TN is a reversed-phase HPLC column from Dr. Maisch HPLC GmbH, packed with ultra-pure, fully porous spherical silica (300 Å pore size) functionalized with trifunctionally bonded butyl ligands and deliberately not endcapped. This design offers mild hydrophobic retention through the C4 ligands, while the absence of endcapping preserves residual silanol activity, enabling additional polar and ionic interactions. The wide 300 Å pore size ensures efficient mass transfer for proteins, peptides, and other large biomolecules, while the unique mixed-mode selectivity allows the retention and separation of analytes that are challenging on conventional endcapped reversed-phase columns.
Reprospher 300 C4-TN from Dr. Maisch HPLC GmbH combines robust reversed-phase performance with enhanced polar selectivity – the ideal solution for laboratories working on complex biomolecules and advanced method development.
Trifunctional C4 bonding without endcapping – USP L26
300 Å pore size optimized for proteins and high-molecular-weight analytes
Mild hydrophobic retention with additional silanol interactions
Mixed-mode selectivity for enhanced method development
Manufactured and QC-tested by Dr. Maisch HPLC GmbH
Available in 5 µm
Separation of proteins, glycoproteins, and large peptides
Mixed-mode retention of polar or basic biomolecules
Method development requiring controlled silanol activity
Bioanalytical workflows for therapeutic proteins and biopharmaceuticals
| Particle Size |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | C4 |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | No |
| Carbonload | 4 % |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| Reprospher 300 C4-TN, 5 µm | rs35.4tn. |