Reprospher 300 C8 is a reversed-phase HPLC column from Dr. Maisch HPLC GmbH, packed with high-purity, fully porous silica featuring a 300 Å pore size and bonded with octyl ligands. The combination of wide-pore architecture and moderate hydrophobicity enables fast, reproducible separation of large, lipophilic biomolecules such as peptides, protein fragments, and antibody subunits. Its reduced retention compared to C18 phases makes it particularly suitable for analytes that require faster elution or exhibit excessive retention on longer-chain columns.
This wide-pore octyl phase from Dr. Maisch HPLC GmbH delivers reliable performance and optimized retention — ideal for reversed-phase separations of hydrophobic peptides and mid-sized proteins.
Octyl (C8) bonded reversed-phase phase – USP L7 classification
Fully porous silica with 300 Å pore size for high-molecular-weight analytes
Balanced hydrophobicity for faster elution of strongly retained compounds
Excellent peak shape and reproducibility under acidic and neutral pH
Available in 1.8 µm, 2 µm, 3 µm and 5 µm particle sizes
Peptides, glycopeptides, and protein domains
Hydrophobic biomolecules and lipophilic drug conjugates
Reversed-phase LC-MS and UV-based bioanalysis
Method development for large molecules requiring moderate retention
| Particle Sizes |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | C8 |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | Yes |
| Carbonload | 4 % |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| Reprospher 300 C8, 1.8 µm | rs318.8e. |
| Reprospher 300 C8, 2 µm | rs32.8e. |
| Reprospher 300 C8, 3 µm | rs33.8e. |
| Reprospher 300 C8, 5 µm | rs35.8e. |