Reprospher 300 C8-T

Trifunctional C8 Reversed-Phase Column for Biomolecule and Peptide Analysis

Reprospher 300 C8-T is a high-performance reversed-phase HPLC column from Dr. Maisch HPLC GmbH, packed with ultra-pure, fully porous spherical silica (300 Å pore size) functionalized with trifunctionally bonded octyl ligands. The medium-chain C8 bonding provides moderate hydrophobic retention, making it ideal for analytes that are over-retained on C18 but still require stronger interaction than C4. The wide pore size (300 Å) ensures efficient access and mass transfer for peptides, proteins, and other large biomolecules, while the trifunctional bonding chemistry delivers enhanced stability, reproducibility, and extended column lifetime under demanding analytical conditions.

Reprospher 300 C8-T from Dr. Maisch HPLC GmbH offers robust reversed-phase performance with balanced hydrophobicity – the ideal choice for laboratories working with biomolecules and peptides in pharmaceutical and biotechnological analysis.

Key Features:

  • Trifunctional C8 bonding on spherical silica – USP L7

  • 300 Å pore size optimized for peptides, proteins, and biomolecules

  • Moderate hydrophobic retention for balanced selectivity

  • High chemical and mechanical stability for reproducible performance

  • Manufactured and QC-tested by Dr. Maisch HPLC GmbH

  • Available in 5 µm

Typical Applications:

  • Reversed-phase HPLC of peptides, proteins, and oligonucleotides

  • Bioanalytical separations requiring moderate retention strength

  • Gradient methods for biomolecule profiling and impurity analysis

  • Method development where C18 is too retentive and C4 too weak

Specifications

Request Product
Particle Size
  • 5 µm
Pore Size 300 Å
Surface Area 100 m²/g
Phase/Modification C8
Particle Type
Separation Mode
USP Code
Endcapping Yes
Carbonload 6 %
pH Range 2 to 8

Material Number

Phase Material Number
Reprospher 300 C8-T, 5 µm rs35.8t.
Reprospher 300 C8 Reprospher 300 C8-TN

Any questions?
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info@dr-maisch.com +49 7073 5035 7

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